BT31010: Plant Cell Tissue Culture
| BT31010 | |||||||||||||||||||||||||||
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| Course name | Plant Cell Tissue Culture | ||||||||||||||||||||||||||
| Offered by | Biotechnology | ||||||||||||||||||||||||||
| Credits | 4 | ||||||||||||||||||||||||||
| L-T-P | 3-1-0 | ||||||||||||||||||||||||||
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| Semester | Spring | ||||||||||||||||||||||||||
Syllabus
Syllabus mentioned in ERP
Fundamentals of plant tissue culture, plant regeneration: organogenesis. Somatic embryogenesis; somaclonal variation, its genetic basis and application in crop improvement. Cell/callus line selection for resistance to herbicide, stress and diseases. Role of tissue culture in rapid clonal propagation, production of pathogen - free plants and "synthetic seeds"; haploid production: advantages and methods. Protoplast technology: isolation, culture and plant regeneration, protoplast fusion, identification and characterization of somatic hybrids, applications of protoplast technology. Specific gene transfer: indirect and direct methods, current status and limitations. Automation in plant tissue culture. Field techniques for propagation of regenerated plants.