BT41012: Downstream Processing
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| BT41012 | |||||||||||||||||||||||||||
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| Course name | Downstream Processing | ||||||||||||||||||||||||||
| Offered by | Biotechnology | ||||||||||||||||||||||||||
| Credits | 3 | ||||||||||||||||||||||||||
| L-T-P | 3-0-0 | ||||||||||||||||||||||||||
| Previous Year Grade Distribution | |||||||||||||||||||||||||||
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| Semester | Spring | ||||||||||||||||||||||||||
Syllabus
Syllabus mentioned in ERP
Introduction; An overview of bioseparation. Separation of cells and other insolubles from fermented broth. Filtration and microfiltration, centrifugation (batch, continuous, basket). Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods (alkali, detergents), Enzymatic methods Products isolation: Extraction and adsorption method, precipitation (ammonium sulphate. Organic solvents, high molecular weight polymers), column chromatography; ultrafiltration., Products polishing: Crystallization and drying.