BT41012: Downstream Processing
Jump to navigation
Jump to search
| BT41012 | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Course name | Downstream Processing | ||||||||||||||||||||||||||
| Offered by | Biotechnology | ||||||||||||||||||||||||||
| Credits | 3 | ||||||||||||||||||||||||||
| L-T-P | 3-0-0 | ||||||||||||||||||||||||||
| Previous Year Grade Distribution | |||||||||||||||||||||||||||
| |||||||||||||||||||||||||||
| Semester | Spring | ||||||||||||||||||||||||||
Syllabus[edit | edit source]
Syllabus mentioned in ERP[edit | edit source]
Introduction; An overview of bioseparation. Separation of cells and other insolubles from fermented broth. Filtration and microfiltration, centrifugation (batch, continuous, basket). Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods (alkali, detergents), Enzymatic methods Products isolation: Extraction and adsorption method, precipitation (ammonium sulphate. Organic solvents, high molecular weight polymers), column chromatography; ultrafiltration., Products polishing: Crystallization and drying.